"diafiltered" meaning in English

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          "text": "For isolation of extracellular fraction, the culture was centrifuged at 2100×g for 5 min and the cell free medium was concentrated and diafiltered by Vivaspin-20 (30 kDa cutoff) sample concentrator (GE Healthcare, UK).",
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        "simple past and past participle of diafilter"
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          "text": "For isolation of extracellular fraction, the culture was centrifuged at 2100×g for 5 min and the cell free medium was concentrated and diafiltered by Vivaspin-20 (30 kDa cutoff) sample concentrator (GE Healthcare, UK).",
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