"diafiltered" meaning in English

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  "lang_code": "en",
  "pos": "verb",
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          "ref": "2016 January 26, “Cloning and Expression of Phytase appA Gene from Shigella sp. CD2 in Pichia pastoris and Comparison of Properties with Recombinant Enzyme Expressed in E . coli”, in PLOS ONE, →DOI",
          "text": "For isolation of extracellular fraction, the culture was centrifuged at 2100×g for 5 min and the cell free medium was concentrated and diafiltered by Vivaspin-20 (30 kDa cutoff) sample concentrator (GE Healthcare, UK).",
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        "simple past and past participle of diafilter"
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          "text": "For isolation of extracellular fraction, the culture was centrifuged at 2100×g for 5 min and the cell free medium was concentrated and diafiltered by Vivaspin-20 (30 kDa cutoff) sample concentrator (GE Healthcare, UK).",
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